Pepstatin waa pentapeptide, oo si dabiici ah u dhacda aspartyl protease inhibitor, kaas oo joojin kara protease aspartic iyo borotiinka aashitada ee noolaha kala duwan.Pepstatin waxaa inta badan soo saara noocyada Streptomyces waxaana soo saaray Streptomyces.Waxay joojin kartaa pepsin, pepsin D iyo angiotensin-sii daaya enzyme, waxayna leedahay saameyn daweyn ah boogaha gastric, hypertension kelyaha, arthritis, bararka carrageenan iyo cuduro kale.
Pepstatin waa ka hortag adag oo ah borotiinka aspartyl sida pepsin, cathepsin D, iyo renin.Pentapeptide-ka dabiiciga ah ee ka go'doonsan actinomycetes wuxuu ahaa sanado badan renin inhibitor-ka caadiga ah ee vitro.Pepstatin maaha mid u gaar ah renin oo si liidata ugu milmay biyaha.Qaybaha qaab dhismeedka ee pepstatin waxa ay kordhisay milanka iyo gaar ahaaneed ee renin dhawr amar oo waaweyn U gudub xaalad kala guurka peptide bond hydrolysis by aspartyl proteases.
Marka borotiinka laga soo saaro unugyada jaban, borotiinku waa la sii deyn karaa, kuwaas oo u baahan in si degdeg ah loo xakameeyo si borotiinka looga ilaaliyo inuu hoos u dhaco.Habka soo saarista borotiinka, protease inhibitors waxay u baahan yihiin in lagu daro si looga hortago proteolysis.Protease inhibitor si guud waxa loola jeedaa walax ku xidha kooxaha qaar xarunta firfircoon ee molecules protease, si ay hawsha protease hoos u dhacdo ama xataa u baaba'do, laakiin ma diido borotiinka enzyme.Dareenka borotiinka kala duwan ee borotiinka kala duwan waa ka duwan yahay, sidaas darteed waa lagama maarmaan in la hagaajiyo xoojinta borotiinka kala duwan.Sababtoo ah milanka protease inhibitor ee dareeraha ayaa aad u hooseeya, waa in fiiro gaar ah loo yeesho in ka hortagga protease in si buuxda loogu daro kaydiyaha si loo yareeyo roobabka protease inhibitor.Pepstantin A wuxuu joojin karaa borotiinka aashitada sida pepsin, angiotensin, collagenase, cathepsin D iyo chymosin.
Pepstatin A waa xakameynta cathepsin d iyo e.Ka dib markii unugyada HEK293 lagu daaweeyay uruurin kala duwan oo pepstatin A ah 24 saacadood, muujinta LC3Ⅱ iyo p62 ayaa la ogaaday.Natiijooyinka waxay muujiyeen in pepstatin A ay si weyn u wanaajin karto muujinta LC3Ⅱ iyo P62 (P<0.05) qaab ku tiirsan qiyaasta.20μ g / ml pepstatin A ayaa loo isticmaalay in lagu daaweeyo HEK293 waqtiyo kala duwan, iyo saameynta waqtiyada kala duwan ee muujinta LC3II iyo p62 ayaa la arkay.Natiijooyinku waxay muujiyeen in pepstatin A ay kor u qaadi karto muujinta LC3II iyo p62 si waqti-ku-tiirsane ah.
Waxaan nahay soo saaraha polypeptide ee Shiinaha, oo leh dhowr sano oo waayo-aragnimo ah oo bislaaday wax soo saarka polypeptide.Hangzhou Taijia Biotech Co., Ltd. waa xirfadle soo saaraha alaabta ceeriin polypeptide, kaas oo ku siin kara tobanaan kun oo polypeptide alaabta ceeriin iyo sidoo kale loo habayn karaa iyadoo loo eegayo baahiyaha.Tayada alaabada polypeptide waa mid aad u fiican, nadiifintuna waxay gaari kartaa 98%, taas oo ay aqoonsadeen isticmaalayaasha adduunka oo dhan. Ku soo dhawow inaad nala tashato.
